Publications

2016

Coombes C, Yamamoto A, McClellan M, Reid TA, Plooster M, Luxton GW, Alper J, Howard J, Gardner MK: Mechanism of microtubule lumen entry for the α-tubulin acetyltransferase enzyme αTAT1.. PNAS. 2016 Nov 1; PMID: 27803321
Sartori P, Geyer VF, Howard J, Jülicher F: Curvature regulation of the ciliary beat through axonemal twist. Phys. Rev. E, 2016 Oct 28;  PMID: 27841522
Xiao X, Geyer VF, Bowne-Anderson H, Howard J, Sbalzarini IF: Automatic optimal filament segmentation with sub-pixel accuracy using generalized linear models and B-spline level-sets. Med Image Anal. 2016 Aug; 2016 Apr 4. PMID: 27104582
Garzon-Coral C, Fantana HA, Howard J: A force-generating machinery maintains the spindle at the cell center during mitosis. Science. 2016 May 27. PMID: 27230381
Sartori P, Geyer VF, Scholich A, Jülicher F, Howard J: Dynamic curvature regulation accounts for the symmetric and asymmetric beats of Chlamydomonas flagella. Elife. 2016 May 11; 2016 May 11. PMID: 27166516
Battle C, Broedersz CP, Fakhri N, Geyer VF, Howard J, Schmidt CF, MacKintosh FC: Broken detailed balance at mesoscopic scales in active biological systems. Science. 2016 Apr 29. PMID: 27126047
Geyer VF, Sartori P, Friedrich BM, Jülicher F, Howard J: Independent Control of the Static and Dynamic Components of the Chlamydomonas Flagellar Beat. Curr Biol. 2016 Apr 25; 2016 Mar 31. PMID: 27040779
Borisy G, Heald R, Howard J, Janke C, Musacchio A, Nogales E: Microtubules: 50 years on from the discovery of tubulin. Nat Rev Mol Cell Biol. 2016 Apr 22. PMID: 27103327
Carrillo Oesterreich F, Herzel L, Straube K, Hujer K, Howard J, Neugebauer KM: Splicing of Nascent RNA Coincides with Intron Exit from RNA Polymerase II. Cell. 2016 Apr 7; 2016 Mar 24. PMID: 27020755

2015

Bowne-Anderson H, Hibbel A, Howard J: Regulation of Microtubule Growth and Catastrophe: Unifying Theory and Experiment. Trends Cell Biol. 2015 Dec. PMID: 26616192
Hibbel A, Bogdanova A, Mahamdeh M, Jannasch A, Storch M, Schäffer E, Liakopoulos D, Howard J: Kinesin Kip2 enhances microtubule growth in vitro through length-dependent feedback on polymerization and catastrophe. Elife. 2015 Nov 18; 2015 Nov 18. PMID: 26576948

2014

Alper JD, Decker F, Agana B, Howard J: The motility of axonemal dynein is regulated by the tubulin code. Biophys J. 2014 Dec 16. PMID: 25658008
Liang X, Madrid J, Howard J: The microtubule-based cytoskeleton is a component of a mechanical signaling pathway in fly campaniform receptors. http://ncbi.nlm.nih.gov/pubmed?term=25517144
Howard J: Quantitative cell biology: the essential role of theory. Mol Biol Cell. 2014 Nov 5. PMID: 25368416
Howard J: Jonathon Howard: motor proteins go walkabout. Interview by Caitlin Sedwick. J Cell Biol. 2014 Jan 20. PMID: 24446480

2013

Howard J, McIntosh JR, Odde DJ: A brief scientific biography of Prof. Alan J. Hunt. Cell Mol Bioeng. 2013 Dec. PMID: 24683426
Takeshita N, Mania D, Herrero S, Ishitsuka Y, Nienhaus GU, Podolski M, Howard J, Fischer R: The cell-end marker TeaA and the microtubule polymerase AlpA contribute to microtubule guidance at the hyphal tip cortex of Aspergillus nidulans to provide polarity maintenance. J Cell Sci. 2013 Dec 1; 2013 Oct 7. PMID: 24101725
Geyer VF, Jülicher F, Howard J, Friedrich BM: Cell-body rocking is a dominant mechanism for flagellar synchronization in a swimming alga. Proc Natl Acad Sci U S A. 2013 Nov 5; 2013 Oct 21. PMID: 24145440
Trushko A, Schäffer E, Howard J: The growth speed of microtubules with XMAP215-coated beads coupled to their ends is increased by tensile force. Proc Natl Acad Sci U S A. 2013 Sep 3; 2013 Aug 20. PMID: 23964126
Reber SB, Baumgart J, Widlund PO, Pozniakovsky A, Howard J, Hyman AA, Jülicher F: XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules. Nat Cell Biol. 2013 Sep; 2013 Aug 25. PMID: 23974040
Jannasch A, Bormuth V, Storch M, Howard J, Schäffer E: Kinesin-8 is a low-force motor protein with a weakly bound slip state. Biophys J. 2013 Jun 4. PMID: 23746518
Zanic M, Widlund PO, Hyman AA, Howard J: Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels. Nat Cell Biol. 2013 Jun; 2013 May 12. PMID: 23666085
Liang X, Madrid J, Gärtner R, Verbavatz JM, Schiklenk C, Wilsch-Bräuninger M, Bogdanova A, Stenger F, Voigt A, Howard J: A NOMPC-dependent membrane-microtubule connector is a candidate for the gating spring in fly mechanoreceptors. Curr Biol. 2013 May 6; 2013 Apr 11. PMID: 23583554
Bowne-Anderson H, Zanic M, Kauer M, Howard J: Microtubule dynamic instability: a new model with coupled GTP hydrolysis and multistep catastrophe. Bioessays. 2013 May; 2013 Mar 27. PMID: 23532586
Gardner MK, Zanic M, Howard J: Microtubule catastrophe and rescue. Curr Opin Cell Biol. 2013 Feb; 2012 Oct 22. PMID: 23092753
Alper J, Geyer V, Mukundan V, Howard J: Reconstitution of flagellar sliding. Methods Enzymol. 2013. PMID: 23498749

2012

Friel CT, Howard J: Coupling of kinesin ATP turnover to translocation and microtubule regulation: one engine, many machines. J Muscle Res Cell Motil. 2012 Dec; 2012 Mar 24. PMID: 22447431
Leduc C, Padberg-Gehle K, Varga V, Helbing D, Diez S, Howard J: [How to avoid the formation of molecular motors traffic jam during intracellular transport]. Med Sci (Paris). 2012 Dec; 2012 Dec 21. PMID: 23290405
Widlund PO, Podolski M, Reber S, Alper J, Storch M, Hyman AA, Howard J, Drechsel DN: One-step purification of assembly-competent tubulin from diverse eukaryotic sources. Mol Biol Cell. 2012 Nov; 2012 Sep 19. PMID: 22993214
Senthilan PR, Piepenbrock D, Ovezmyradov G, Nadrowski B, Bechstedt S, Pauls S, Winkler M, Möbius W, Howard J, Göpfert MC: Drosophila auditory organ genes and genetic hearing defects. Cell. 2012 Aug 31. PMID: 22939627
Bormuth V, Nitzsche B, Ruhnow F, Mitra A, Storch M, Rammner B, Howard J, Diez S: The highly processive kinesin-8, Kip3, switches microtubule protofilaments with a bias toward the left. Biophys J. 2012 Jul 3. PMID: 22828351
Leduc C, Padberg-Gehle K, Varga V, Helbing D, Diez S, Howard J: Molecular crowding creates traffic jams of kinesin motors on microtubules. Proc Natl Acad Sci U S A. 2012 Apr 17; 2012 Mar 19. PMID: 22431622
Segal M, Soifer I, Petzold H, Howard J, Elbaum M, Reiner O: Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon. Biol Open. 2012 Mar 15; 2012 Jan 6. PMID: 23213412
Tropini C, Roth EA, Zanic M, Gardner MK, Howard J: Islands containing slowly hydrolyzable GTP analogs promote microtubule rescues. PLoS One. 2012; 2012 Jan 17. PMID: 22272281

2011

Gardner MK, Zanic M, Gell C, Bormuth V, Howard J: Depolymerizing kinesins Kip3 and MCAK shape cellular microtubule architecture by differential control of catastrophe. Cell. 2011 Nov 23. PMID: 22118464
Alper J, Howard J: Hybrid four-headed myosin motor engineered with antagonistic motor domains. Proc Natl Acad Sci U S A. 2011 Sep 20; 2011 Sep 7. PMID: 21900606
Friel CT, Howard J: The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization. EMBO J. 2011 Aug 26; 2011 Aug 26. PMID: 21873978
Gardner MK, Charlebois BD, Jánosi IM, Howard J, Hunt AJ, Odde DJ: Rapid microtubule self-assembly kinetics. Cell. 2011 Aug 19. PMID: 21854983
Howard J, Grill SW, Bois JS: Turing's next steps: the mechanochemical basis of morphogenesis. Nat Rev Mol Cell Biol. 2011 Jun. PMID: 21602907
Widlund PO, Stear JH, Pozniakovsky A, Zanic M, Reber S, Brouhard GJ, Hyman AA, Howard J: XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region. Proc Natl Acad Sci U S A. 2011 Feb 15; 2011 Jan 31. PMID: 21282620
Bormuth V, Zörgibel F, Schäffer E, Howard J: Functional surface attachment in a sandwich geometry of GFP-labeled motor proteins. Methods Mol Biol. 2011. PMID: 21809197
Friel CT, Bagshaw CR, Howard J: Analysing the ATP turnover cycle of microtubule motors. Methods Mol Biol. 2011. PMID: 21773929
Gell C, Friel CT, Borgonovo B, Drechsel DN, Hyman AA, Howard J: Purification of tubulin from porcine brain. Methods Mol Biol. 2011. PMID: 21773918
Mayr MI, Storch M, Howard J, Mayer TU: A non-motor microtubule binding site is essential for the high processivity and mitotic function of kinesin-8 Kif18A. PLoS One. 2011; 2011 Nov 10. PMID: 22102900

2010

Khairy K, Foo J, Howard J: Shapes of Red Blood Cells: Comparison of 3D Confocal Images with the Bilayer-Couple Model. Cell Mol Bioeng. 2010 Sep 1. PMID: 21031149
Redemann S, Pecreaux J, Goehring NW, Khairy K, Stelzer EH, Hyman AA, Howard J: Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos. PLoS One. 2010 Aug 20; 2010 Aug 20. PMID: 20808841
Sanhaji M, Friel CT, Kreis NN, Krämer A, Martin C, Howard J, Strebhardt K, Yuan J: Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1. Mol Cell Biol. 2010 Jun; 2010 Apr 5. PMID: 20368358
Nitzsche B, Bormuth V, Bräuer C, Howard J, Ionov L, Kerssemakers J, Korten T, Leduc C, Ruhnow F, Diez S: Studying kinesin motors by optical 3D-nanometry in gliding motility assays. Methods Cell Biol. 2010. PMID: 20466139
Gell C, Bormuth V, Brouhard GJ, Cohen DN, Diez S, Friel CT, Helenius J, Nitzsche B, Petzold H, Ribbe J, Schäffer E, Stear JH, Trushko A, Varga V, Widlund PO, Zanic M, Howard J: Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell Biol. 2010. PMID: 20466138

2009

Kamal MM, Mills D, Grzybek M, Howard J: Measurement of the membrane curvature preference of phospholipids reveals only weak coupling between lipid shape and leaflet curvature. Proc Natl Acad Sci U S A. 2009 Dec 29; 2009 Dec 23. PMID: 20080790
Zanic M, Stear JH, Hyman AA, Howard J: EB1 recognizes the nucleotide state of tubulin in the microtubule lattice. PLoS One. 2009 Oct 23; 2009 Oct 23. PMID: 19851462
Varga V, Leduc C, Bormuth V, Diez S, Howard J: Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization. Cell. 2009 Sep 18. PMID: 19766569
Bormuth V, Varga V, Howard J, Schäffer E: Protein friction limits diffusive and directed movements of kinesin motors on microtubule. Science. 2009 Aug 14. PMID: 19679813
Howard J, Hyman AA: Growth, fluctuation and switching at microtubule plus ends.. Nat Rev Mol Cell Biol. 2009 Aug; 2009 Jun 10. PMID: 19513082
Howard J: Mechanical signaling in networks of motor and cytoskeletal proteins. Annu Rev Biophys. 2009. PMID: 19416067

2008

Crevenna AH, Madathil S, Cohen DN, Wagenbach M, Fahmy K, Howard J: Secondary structure and compliance of a predicted flexible domain in kinesin-1 necessary for cooperation of motors. Biophys J. 2008 Dec; 2008 Sep 5. PMID: 18775962
Bechstedt S, Howard J: Hearing mechanics: a fly in your ear. Curr Biol. 2008 Sep 23. PMID: 18812084
Bormuth V, Jannasch A, Ander M, van Kats CM, van Blaaderen A, Howard J, Schäffer E: Optical trapping of coated microspheres. Opt Express. 2008 Sep 1. PMID: 18772994
Khairy K, Howard J: Spherical harmonics-based parametric deconvolution of 3D surface images using bending energy minimization. Med Image Anal. 2008 Apr; 2007 Oct 30. PMID: 18055248
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA: XMAP215 is a processive microtubule polymerase. Cell. 2008 Jan 11. PMID: 18191222

2007

Elie-Caille C, Severin F, Helenius J, Howard J, Muller DJ, Hyman AA: Straight GDP-tubulin protofilaments form in the presence of taxol. Curr Biol. 2007 Oct 23; 2007 Oct 4. PMID: 17919908
Riedel-Kruse IH, Hilfinger A, Howard J, Jülicher F: How molecular motors shape the flagellar beat. HFSP J. 2007 Sep. PMID: 19404446
Leduc C, Ruhnow F, Howard J, Diez S: Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors. Proc Natl Acad Sci U S A. 2007 Jun 26; 2007 Jun 14. PMID: 17569782
Howard J, Tolić-Nørrelykke IM: Bundling, sliding, and pulling microtubules in cells and in silico. HFSP J. 2007 May; 2007 May 21. PMID: 19404456
Bormuth V, Howard J, Schäffer E: LED illumination for video-enhanced DIC imaging of single microtubules. J Microsc. 2007 Apr. PMID: 17381703
Schäffer E, Nørrelykke SF, Howard J: Surface forces and drag coefficients of microspheres near a plane surface measured with optical tweezers. Langmuir. 2007 Mar 27; 2007 Feb 28. PMID: 17326669
Howard J, Hyman AA: Microtubule polymerases and depolymerases. Curr Opin Cell Biol. 2007 Feb; 2006 Dec 20. PMID: 17184986
Bechstedt S, Howard J: Models of hair cell mechanotransduction. Curr Top Membr. 2007; 2007 Apr 17. PMID: 25168144

2006

Pecreaux J, Röper JC, Kruse K, Jülicher F, Hyman AA, Grill SW, Howard J: Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Curr Biol. 2006 Nov 7. PMID: 17084695
Kerssemakers J, Howard J, Hess H, Diez S: The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy. The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J: Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nat Cell Biol. 2006 Sep; 2006 Aug 13. PMID: 16906145
Dinu CZ, Opitz J, Pompe W, Howard J, Mertig M, Diez S: Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules. Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules
Howard J: Protein power strokes. Curr Biol. 2006 Jul 25. PMID: 16860722
Helenius J, Brouhard G, Kalaidzidis Y, Diez S, Howard J: The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature. 2006 May 4. PMID: 16672973

2005

Riedel IH, Kruse K, Howard J: A self-organized vortex array of hydrodynamically entrained sperm cells. Science. 2005 Jul 8. PMID: 16002619
Poole K, Khairy K, Friedrichs J, Franz C, Cisneros DA, Howard J, Mueller D: Molecular-scale topographic cues induce the orientation and directional movement of fibroblasts on two-dimensional collagen surfaces. J Mol Biol. 2005 Jun 3; 2005 Apr 9. PMID: 15890202

2004

Jiang F, Hörber H, Howard J, Müller DJ: Assembly of collagen into microribbons: effects of pH and electrolytes. J Struct Biol. 2004 Dec. PMID: 15522775
Lawrence CJ, Dawe RK, Christie KR, Cleveland DW, Dawson SC, Endow SA, Goldstein LS, Goodson HV, Hirokawa N, Howard J, Malmberg RL, McIntosh JR, Miki H, Mitchison TJ, Okada Y, Reddy AS, Saxton WM, Schliwa M, Scholey JM, Vale RD, Walczak CE, Wordeman L: A standardized kinesin nomenclature. J Cell Biol. 2004 Oct 11. PMID: 15479732
Haeberle H, Fujiwara M, Chuang J, Medina MM, Panditrao MV, Bechstedt S, Howard J, Lumpkin EA: Molecular profiling reveals synaptic release machinery in Merkel cells. Proc Natl Acad Sci U S A. 2004 Oct 5; 2004 Sep 24. PMID: 15448211
Jiang F, Khairy K, Poole K, Howard J, Müller DJ: Creating nanoscopic collagen matrices using atomic force microscopy. Microsc Res Tech. 2004 Aug. PMID: 15549696
Sawhney RK, Howard J: Molecular dissection of the fibroblast-traction machinery. Cell Motil Cytoskeleton. 2004 Jul. PMID: 15146536
Schief WR, Clark RH, Crevenna AH, Howard J: Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism. Proc Natl Acad Sci U S A. 2004 Feb 3; 2004 Jan 20. PMID: 14734813

2003

Grill SW, Howard J, Schäffer E, Stelzer EH, Hyman AA: The distribution of active force generators controls mitotic spindle position. Science. 2003 Jul 25. PMID: 12881570
Hunter AW, Caplow M, Coy DL, Hancock WO, Diez S, Wordeman L, Howard J: The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends. Mol Cell. 2003 Feb. PMID: 12620232

2002

Sawhney RK, Howard J: Slow local movements of collagen fibers by fibroblasts drive the rapid global self-organization of collagen gels. J Cell Biol. 2002 Jun 10; 2002 Jun 10. PMID: 12058022
Vinh DB, Kern JW, Hancock WO, Howard J, Davis TN: Reconstitution and characterization of budding yeast gamma-tubulin complex. Mol Biol Cell. 2002 Apr. PMID: 11950928
Diez S, Schief WR, Howard J: Molecular motors: single-molecule recordings made easy. Curr Biol. 2002 Mar 19. PMID: 11909546